| Active Players |
| 11 | Aiyuk, Brandon | WR | 03/17/1998 | 24 | 6' 00" | 200 | Arizona State | SF | 20 01 025 | 3 |
| 51 | Al-Shaair, Azeez | LB | 08/04/1997 | 25 | 6' 02" | 228 | Florida Atlantic | SF FA | 19 CFA | 4 |
| 91 | Armstead, Arik | DT | 11/15/1993 | 28 | 6' 07" | 290 | Oregon | SF | 15 01 017 | 8 |
| 65 | Banks, Aaron | OG | 09/03/1997 | 25 | 6' 05" | 325 | Notre Dame | SF | 21 02 048 | 2 |
| 97 | Bosa, Nick | DE | 10/23/1997 | 24 | 6' 04" | 266 | Ohio State | SF | 19 01 002 | 4 |
| 64 | Brendel, Jake | OC | 09/10/1992 | 30 | 6' 04" | 299 | UCLA | DAL FA | 16 CFA | 6 |
| 60 | Brunskill, Daniel | OG/OC | 01/27/1994 | 28 | 6' 05" | 300 | San Diego State | ATL FA | 17 CFA | 4 |
| 74 | Burford, Spencer | OG | 07/19/2000 | 22 | 6' 04" | 300 | UTSA | SF | 22 04 134 | R |
| 48 | Burks, Oren | LB | 03/21/1995 | 27 | 6' 03" | 233 | Vanderbilt | GB | 18 03 088 | 5 |
| 32 | Davis-Price, Tyrion | RB | 10/23/2000 | 21 | 6' 00" | 219 | LSU | SF | 22 03 093 | R |
| 82 | Dwelley, Ross | TE | 01/26/1995 | 27 | 6' 05" | 235 | San Diego | SF FA | 18 CFA | 5 |
| 56 | Ebukam, Samson | DE | 05/09/1995 | 27 | 6' 01" | 245 | Eastern Washington | LAR | 17 04 125 | 6 |
| 45 | Flannigan-Fowles, Demetrius | LB | 09/04/1996 | 26 | 6' 02" | 223 | Arizona | SF FA | 19 CFA | 3 |
| 10 | Garoppolo, Jimmy | QB | 11/02/1991 | 30 | 6' 02" | 225 | Eastern Illinois | NE | 14 02 062 | 9 |
| 31 | Gipson Sr., Tashaun | FS | 08/07/1990 | 32 | 6' 01" | 212 | Wyoming | CLE FA | 12 CFA | 11 |
| 90 | Givens, Kevin | DT | 03/01/1997 | 25 | 6' 01" | 285 | Penn State | SF FA | 19 CFA | 3 |
| 9 | Gould, Robbie | PK | 12/30/1981 | 40 | 6' 00" | 190 | Penn State | NE FA | 05 CFA | 18 |
| 6 | Gray, Danny | WR | 04/01/1999 | 23 | 6' 00" | 200 | SMU | SF | 22 03 105 | R |
| 57 | Greenlaw, Dre | LB | 05/25/1997 | 25 | 5' 11" | 230 | Arkansas | SF | 19 05 148 | 4 |
| 61 | Hance, Blake | OG | 01/11/1996 | 26 | 6' 06" | 310 | Northwestern | BUF FA | 19 CFA | 2 |
| 29 | Hufanga, Talanoa | SS | 02/02/1999 | 23 | 6' 01" | 200 | USC | SF | 21 05 180 | 2 |
| 92 | Hyder Jr., Kerry | DE | 05/02/1991 | 31 | 6' 02" | 275 | Texas Tech | NYJ FA | 14 CFA | 7 |
| 95 | Jackson, Drake | DE | 04/12/2001 | 21 | 6' 04" | 273 | USC | SF | 22 02 062 | R |
| 15 | Jennings, Jauan | WR | 07/10/1997 | 25 | 6' 03" | 212 | Tennessee | SF | 20 07 217 | 2 |
| 44 | Juszczyk, Kyle | FB | 04/23/1991 | 31 | 6' 01" | 235 | Harvard | BAL | 13 04 130 | 10 |
| 99 | Kinlaw, Javon | DT | 10/03/1997 | 25 | 6' 05" | 319 | South Carolina | SF | 20 01 014 | 3 |
| 85 | Kittle, George | TE | 10/09/1993 | 29 | 6' 04" | 250 | Iowa | SF | 17 05 146 | 6 |
| 81 | Kroft, Tyler | TE | 10/15/1992 | 29 | 6' 05" | 252 | Rutgers | CIN | 15 03 085 | 8 |
| 38 | Lenoir, Deommodore | CB | 10/06/1999 | 23 | 5' 10" | 200 | Oregon | SF | 21 05 172 | 2 |
| 23 | Mack, Marlon | RB | 03/07/1996 | 26 | 5' 11" | 212 | South Florida | IND | 17 04 43 | 6 |
| 24 | Mason, Jordan | RB | 05/24/1999 | 23 | 5' 11" | 223 | Georgia Tech | SF FA | 22 CFA | R |
| 3 | McCloud III, Ray-Ray | WR | 10/15/1996 | 25 | 6' 03" | 190 | Clemson | BUF | 18 06 187 | 5 |
| 69 | McGlinchey, Mike | OT | 01/12/1995 | 27 | 6' 08" | 310 | Notre Dame | SF | 18 01 009 | 5 |
| 68 | McKivitz, Colton | OT | 08/09/1996 | 26 | 6' 06" | 301 | West Virginia | SF | 20 05 153 | 3 |
| 76 | Moore, Jaylon | OT/OG | 01/09/1998 | 24 | 6' 04" | 311 | Western Michigan | SF | 21 05 155 | 2 |
| 33 | Moore, Tarvarius | S | 08/16/1996 | 26 | 6' 04" | 200 | Southern Miss | SF | 18 03 095 | 5 |
| 4 | Moseley, Emmanuel | CB | 03/25/1996 | 26 | 5' 11" | 190 | Tennessee | SF FA | 18 CFA | 5 |
| 30 | Odum, George | S | 11/03/1993 | 28 | 6' 01" | 202 | Central Arkansas | IND FA | 18 CFA | 5 |
| 94 | Omenihu, Charles | DE | 08/20/1997 | 25 | 6' 05" | 280 | Texas | HOU | 19 05 161 | 4 |
| 46 | Pepper, Taybor | LS | 05/28/1994 | 28 | 6' 04" | 245 | Michigan State | GB FA | 17 SFA | 5 |
| 13 | Purdy, Brock | QB | 12/27/1999 | 22 | 6' 01" | 220 | Iowa State | SF | 22 07 262 | R |
| 98 | Ridgeway, Hassan | DT | 11/02/1994 | 27 | 6' 03" | 305 | Texas | IND | 16 04 116 | 7 |
| 19 | Samuel, Deebo | WR | 01/15/1996 | 26 | 5' 11" | 215 | South Carolina | SF | 19 02 036 | 4 |
| 20 | Thomas, Ambry | CB | 09/09/1999 | 23 | 6' 00" | 190 | Michigan | SF | 21 03 102 | 2 |
| 53 | Turay, Kemoko | DE | 07/11/1995 | 27 | 6' 05" | 248 | Rutgers | IND | 18 02 052 | 5 |
| 7 | Ward, Charvarius | CB | 05/16/1996 | 26 | 6' 01" | 196 | Middle Tennessee | DAL FA | 18 CFA | 5 |
| 54 | Warner, Fred | LB | 11/19/1996 | 25 | 6' 03" | 230 | BYU | SF | 18 03 070 | 5 |
| 71 | Williams, Trent | OT | 08/19/1988 | 34 | 6' 05" | 320 | Oklahoma | WAS | 10 01 004 | 13 |
| 22 | Wilson Jr., Jeff | RB | 11/16/1995 | 26 | 6' 00" | 213 | North Texas | SF FA | 18 CFA | 5 |
| 18 | Wishnowsky, Mitch | PT | 03/02/1992 | 30 | 6' 02" | 220 | Utah | SF | 19 04 110 | 4 |
| 89 | Woerner, Charlie | TE | 10/16/1997 | 24 | 6' 05" | 241 | Georgia | SF | 20 06 190 | 3 |
| 26 | Womack III, Samuel | CB | 07/07/1999 | 23 | 5' 09" | 189 | Toledo | SF | 22 05 172 | R |
| 63 | Zakelj, Nick | OG | 06/22/1999 | 23 | 6' 06" | 316 | Fordham | SF | 22 06 187 | R |
| Practice Squad |
| 58 | Barrett, Alex | DE | 03/06/1994 | 28 | 6' 02" | 250 | San Diego State | DET FA | 17 CFA | 2 |
| 14 | Benkert, Kurt | QB | 07/17/1995 | 27 | 6' 03" | 218 | Virginia | ATL FA | 18 CFA | 2 |
| 28 | Coleman, Tevin | RB | 04/16/1993 | 29 | 5' 11" | 210 | Indiana | ATL | 15 03 073 | 8 |
| 88 | Fumagalli, Troy | TE | 02/17/1995 | 27 | 6' 05" | 248 | Wisconsin | DEN | 18 05 156 | 5 |
| 77 | Gutierrez, Alfredo | OT | 12/29/1995 | 26 | 6' 09" | 332 | No College | SF FA | 21 SFA | 1 |
| 41 | Hawkins, Tayler | S | 12/27/1997 | 24 | 6' 01" | 205 | San Diego State | SF FA | 22 CFA | R |
| 67 | Ismael, Keith | OC | 07/25/1998 | 24 | 6' 03" | 310 | San Diego State | WAS | 20 05 156 | 3 |
| 47 | Johnson, Buddy | ILB | 02/27/1999 | 23 | 6' 01" | 240 | Texas A&M | PIT | 21 04 140 | 2 |
| 27 | Johnson, Dontae | CB | 12/01/1991 | 30 | 6' 02" | 190 | North Carolina State | SF | 14 04 129 | 9 |
| 43 | Knight, Qwuantrezz | CB | 10/10/1997 | 24 | 6' 00" | 199 | UCLA | SF FA | 22 CFA | R |
| 86 | Martin, Tay | WR | 12/14/1997 | 24 | 6' 03" | 185 | Oklahoma State | SF FA | 22 CFA | R |
| 40 | McCrary-Ball, Marcelino | LB | 03/23/1999 | 23 | 6' 00" | 214 | Indiana | SF FA | 22 CFA | R |
| 62 | Poe, Jason | OG | 07/27/1998 | 24 | 6' 01" | 300 | Mercer | SF FA | 22 CFA | R |
| 83 | Snead IV, Willie | WR | 10/17/1992 | 29 | 5' 11" | 195 | Ball State | CLE FA | 14 CFA | 8 |
| 55 | Spence, Akeem | DT | 11/29/1991 | 30 | 6' 01" | 307 | Illinois | TB | 13 04 100 | 9 |
| 17 | Turner, Malik | WR | 01/30/1996 | 26 | 6' 02" | 202 | Illinois | SEA FA | 18 CFA | 5 |
| 78 | Watson, Leroy | OT | 06/10/1998 | 24 | 6' 05" | 311 | UTSA | ATL FA | 22 CFA | R |
| Reserves |
| 93 | Davis, Kalia | DT | 10/02/1998 | 24 | 6' 01" | 310 | Central Florida | SF | 22 06 220 | R |
| 96 | Hurst, Maurice | DT | 05/09/1995 | 27 | 6' 01" | 291 | Michigan | OAK | 18 05 140 | 5 |
| 5 | Lance, Trey | QB | 05/09/2000 | 22 | 6' 04" | 224 | North Dakota State | SF | 21 01 003 | 2 |
| 88 | Matthews, Jordan | TE | 07/16/1992 | 30 | 6' 03" | 236 | Vanderbilt | PHI | 14 02 042 | 7 |
| 25 | Mitchell, Elijah | RB | 05/02/1998 | 24 | 5' 10" | 200 | Louisiana | SF | 21 06 194 | 2 |
| 59 | Robinson, Curtis | LB | 06/02/1998 | 24 | 6' 03" | 235 | Stanford | DEN FA | 21 CFA | 2 |
| 78 | Schlueter, Sam | OT | 08/11/1997 | 25 | 6' 06" | 309 | Minnesota | SF FA | 22 CFA | R |
| 2 | Verrett, Jason | CB | 05/13/1991 | 31 | 5' 09" | 188 | TCU | SD | 14 01 025 | 9 |
| 1 | Ward, Jimmie | FS | 07/18/1991 | 31 | 5' 11" | 195 | Northern Illinois | SF | 14 01 030 | 9 |
| 75 | Willis, Jordan | DE | 05/02/1995 | 27 | 6' 04" | 270 | Kansas State | CIN | 17 03 073 | 6 |